Nitrogen Significantly Affected N Cycling Functional Gene Abundances Compared with Phosphorus and Drought in an Alpine Meadow

Human activities and global climate change have greatly increased nitrogen (N) phosphorus (P) inputs altered precipitation patterns in alpine meadows. Functional genes are important indicators of microorganisms that drive the cycling process; however, functional gene responses soil to N P availability drought remain unclear. Separate or combined treatments fertilization were conducted on Zoige Wetland Qinghai-Tibet Plateau, abundances nitrification AOA amoA AOB denitrification nirS, nirK, nosZ measured explore response these treatments. Seven treatments, including control (CK), addition (N), (P), 50% reduction (D), (NP), with (ND), NP (NPD), investigated. The results indicated application significantly abundance, while had no significant effects abundance genes. treatment but did not affect suggesting was more responsive moisture than amoA. However, affected by Denitrification less sensitive integrated addition, above These indicate may play a critical role process ammonia oxidation meadows, (nirK, nosZ) better ammoxidation (AOA AOB) at adapting environmental changes caused increasing deposition