Nicotine dosimetry and stability in cambridge filter PADs (CFPs) following different smoking regime protocols and storage conditions

Despite the growing numbers of studies on cigarettes and electronic nicotine delivery products (ENDs), no standard assessment stability in various matrix post exposure is currently available. The aim present study was to evaluate optimal condition store Cambridge Filter Pads (CFPs) before chemical analysis order guarantee titer nicotine.We further performed data normalization according different smoking or vaping runs. Smoke vapor generated respectively by a reference tobacco cigarette (1R6F) ENDs under regimes (ISO, HCI CRM81) were collected CFPs as total particulate matter (TPM) subsequently analyzed for content. For each exposure, some at time zero, whereas others stored conditions after 30 days. Principal Component Analysis (PCA) showed best correlation between TPM normalization. This suggests that can affect preservation while samples storage −80 °C may prevent loss nicotine. Finally, with strongly recommended regulatory purpose.