Nicotinate Phosphoribosyltransferase of Yeast

Nicotinate phosphoribosyltransferase of yeast. Purification and properties.

Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum.

We have determined the crystal structure of nicotinate phosphoribosyltransferase from Themoplasma acidophilum (TaNAPRTase). The TaNAPRTase has three domains, an N-terminal domain, a central functional domain, and a unique C-terminal domain. The crystal structure revealed that the functional domain has a type II phosphoribosyltransferase fold that may be a common architecture for both nicotinic ...

Crystal Structure of Sus scrofa Quinolinate Phosphoribosyltransferase in Complex with Nicotinate Mononucleotide

We have determined the crystal structure of porcine quinolinate phosphoribosyltransferase (QAPRTase) in complex with nicotinate mononucleotide (NAMN), which is the first crystal structure of a mammalian QAPRTase with its reaction product. The structure was determined from protein obtained from the porcine kidney. Because the full protein sequence of porcine QAPRTase was not available in either ...

Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors.

Nicotinamide adenine dinucleotide (NAD) is a cofactor in redox reactions and a substrate for NAD-consuming enzymes, such as PARPs and sirtuins. As cancer cells have increased NAD requirements, the main NAD salvage enzymes in humans, nicotinamide phosphoribosyltransferase (NAMPT) and nicotinate phosphoribosyltransferase (NAPRT), are involved in the development of novel anti-cancer therapies. Kno...

Baker ’ s Yeast UMP : Pyrophosphate Phosphoribosyltransferase

Baker’s yeast UMP:pyrophosphate phosphoribosyltransferase (EC 2.4.2.9) has been extensively purified and the final preparation seemed to be pure but not homogeneous. The native enzyme showed a molecular weight of 80,000 calculated by gel filtration; disc gel electrophoresis performed in the presence of sodium dodecyl sulfate and reducing agents showed two dissimilar subunits and the sum of thei...