NgoII, a restriction endonuclease from Neisseria gonorrhoeae
نویسندگان
چکیده
منابع مشابه
Differentiation of Neisseria gonorrhoeae from other Neisseria species by use of the restriction endonuclease HaeIII.
We used the restriction endonuclease HaeIII to differentiate Neisseria gonorrhoeae from other Neisseria species and Branhamella catarrhalis. A total of 16 clinical isolates and four American Type Culture Collection strains of N. gonorrhoeae were resistant to HaeIII digestion, whereas 17 isolates and four American Type Culture Collection strains from eight different bacterial species were suscep...
متن کاملElectrophoretic comparison of endonuclease-digested plasmids from Neisseria gonorrhoeae.
In order to associate virulence in Neisseria gonorrhoeae with an alteration of the nucleotide sequence of its small covalently closed plasmid, plasmid deoxyribonucleic acid was isolated from both virulent (T1) and avirulent (T3) morphological types for two strains. Electrophoretic and contour length measurements of intact plasmids indicated a homogeneous population with a molecular weight of ap...
متن کاملNeisseria gonorrhoeae by restriction fragment length polymorphisms
Objective-To characterise Neisseria gonorrhoeae isolates by restriction fragment length polymorphisms (RFLPs) in ribosomal RNA genes. Design-Generation of RFLP patterns by HincII restriction of rRNA genes followed by hybridisation with a nonradioactive labelled broad spectrum 16 + 23S rRNA gene probe. This typing method was developed and compared with MAb based serotyping. Specimens-Forty three...
متن کاملNdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences.
Type II restriction endonucleases recognize and cleave near specific DNA sequences, usually 4-6 basepair palindromes. They are of fundamental importance as tools for the recombinant DNA technology as they provide the selective cleavages required for the analysis and restructuring of DNA in vitro. The flexibility of these techniques is proportional to the number of DNA sequences which can be cle...
متن کاملPreferential uptake of restriction fragments from a gonococcal cryptic plasmid by competent Neisseria gonorrhoeae.
Factors involved in the specificity of DNA uptake by competent Neisseria gonorrhoeae were examined. Host-controlled modification did not affect uptake. Certain restriction fragments of the 4.2 kb gonococcal cryptic plasmid pFA1 and of the replicative form of the bacteriophage M13 were taken up in preference to others, independent of differences in fragment size. A 600 bp fragment from the 4.2 k...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1979
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.137.3.1299-1307.1979