Newsmaker: Biocentury Transgene

Newsmaker of the year: In the eye of the storm.

Jane Lubchenco smiles as a dolphin leaps out of the water, arcs in the air and splashes back down just a few metres away. The 63-year-old marine ecologist is out on a boat near Pascagoula, Mississippi, with a team of researchers studying how the recent oil spill in the Gulf of Mexico has affected dolphin communities there. On this October day, Lubchenco wears starfish-shaped earrings and a cap ...

Transgene inheritance in plants.

The patterns of transgene inheritance in plants and the possible explanations for non-Mendelian transmission are reviewed. The non-Mendelian inheritance of a transgene has been recorded with a frequency between 10% and 50% in transgenic plants produced either by Agrobacterium-mediated transformation or through particle bombardment. Different effects such as deletion, duplication, rearrangement,...

Lentiviral transgene vectors

Transgenic animals can be broadly defined as animals that contain recombinant DNA molecules in their genome that have been introduced by human intervention (Wall, 1996). Developmental studies and clinically relevant disease models are based on transgenic laboratory animals, most commonly the mouse. In farm animals, transgenic technology is used to produce large quantities of pharmaceutically re...

Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene

Synthetic mammalian transgene negative autoregulation

Biological networks contain overrepresented small-scale topologies, typically called motifs. A frequently appearing motif is the transcriptional negative-feedback loop, where a gene product represses its own transcription. Here, using synthetic circuits stably integrated in human kidney cells, we study the effect of negative-feedback regulation on cell-wide (extrinsic) and gene-specific (intrin...