New tool for Gram-negative and Gram-positive bacterial strains differentiation through GCMS direct identification of exogenous VOC metabolites in headspace vials

In this paper, a chromatographic method for the determination of volatile organic compounds (VOCs) as tool Gram-positive and Gram-negative bacterial strains identification was described. Direct Headspace gas chromatography coupled with mass spectrometry (Headspace-GC-MS) has been used qualitative VOCs emitted by certain (Salmonella Typhimurium, Escherichia coli, Serratia rubidae, Staphyloccocus aureus, Enterococcus casseliflavus, faecalis). first stage study, pathogenic bacteria were cultured in broth-specific liquid culture medium directly (lauryl sulfate broth) into headspace vials. Subsequently, VOC emissions analyzed airborne screening Thus, objective to analyze all resulting order select only those compounds, which can be exclusively associated specific bacteria. Qualitative analysis Headspace-GC-MS proven non-invasive, accurate, fast identifying strains, based on emission. A considerable number have determined air, significant difference being observed between cultures compared but also types themselves. The study presents preliminary results, prove that studied is possible, air these cultures. This successfully rapid classical methods.