New semen freezing method for chicken and duck using dimethylacetamide as the cryoprotectant
نویسندگان
چکیده
منابع مشابه
Possible Commercial Method of Freezing Chicken Semen
Introduction Primary breeders are currently faced with the same problem plaguing most animal industries, rising production costs. Labor, feed and energy costs are forcing primary breeders to consider more economical ways of maintaining gene pools. One way to reduce such costs is with the use of frozen semen. With frozen semen available from superior sires, primary breeders will have the tool to...
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BACKGROUND The captive elephant population is not self-sustaining and with a limited number of breeding bulls, its genetic diversity is in decline. One way to overcome this is to import young and healthy animals from the wild. We introduce here a more sustainable alternative method - importation of semen from wild bulls without removing them from their natural habitat. Due to the logistics invo...
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Cryoconservation of blastodermal cells (BCs) can preserve genetic material for the future reconstruction of poultry breeds. The aim of our study was to compare the effects of three slow freezing programs and vitrification, different cryoprotectants (5% DMSO, 10% DMSO, or multi-component cryoprotectant (MC) and two thawing methods on the viability of chicken BCs. Significant differences in the s...
متن کاملSemen cryopreservation for the creation of a Spanish poultry breeds cryobank: optimization of freezing rate and equilibration time.
A sperm cryopreservation protocol requiring dimethylacetamide (DMA, 6%) as a cryoprotectant was optimized via assays involving different prefreezing equilibration times (1, 10, 30, 60, and 120 min at 5°C) and different freezing rates achieved by the following: 1) using nitrogen vapor to reduce the temperature from 5°C to -85°C at 10°C/min (slow freezing rate); 2) using a biological freezer unit...
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در سال های اخیر، تقاضای استفاده از تئوری خطی ویسکوالاستیسیته بیشتر شده است. با افزایش استفاده از کامپوزیت های پیشرفته در صنایع هوایی و همچنین استفاده روزافزون از مواد پلیمری، اهمیت روش های دقیق طراحی و تحلیل چنین ساختارهایی بیشتر شده است. این مواد جدید از خودشان رفتارهای مکانیکی ارائه می دهند که با تئوری های الاستیسیته و ویسکوزیته، نمی توان آن ها را توصیف کرد. این مواد، خواص ویسکوالاستیک دارند....
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2021
ISSN: 0032-5791
DOI: 10.1016/j.psj.2021.101091