New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1
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چکیده
منابع مشابه
Influence of forage phenolics on ruminal fibrolytic bacteria and in vitro fiber degradation.
In vitro cultures of ruminal microorganisms were used to determine the effect of cinnamic acid and vanillin on the digestibility of cellulose and xylan. Cinnamic acid and vanillin depressed in vitro dry matter disappearance of cellulose 14 and 49%, respectively, when rumen fluid was the inoculum. The number of viable Bacteroides succinogenes cells, the predominant cellulolytic organism, was thr...
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This study was done to determine the effectiveness of supplementary enzymes at increasing the fiber digestion by ruminal microorganisms and to assess whether enzyme activity limits the rate of fiber digestion in ruminal digesta. In vitro comparisons of enzyme activities in two feed enzyme preparations (A and B) with enzyme activities extracted from ruminal fluid indicated that the addition of f...
متن کاملIn vitro Effects of Exogenous Fibrolytic Enzymes on Rumen Fermentation of Wild Guinea Grass (Panicum maximum)
This study aimed to evaluate the potential of commercial enzymes cellulase (CE), xylanase (XY) and the combination of CE + XY to improve rumen fermentation of Guinea grass ecotype ‘A’ (Panicum maximum). The experiment was carried out in a randomized complete block design. In vitro incubations were performed with four doses of individual enzymes and their combinations (1:1 from each enzyme) as 5...
متن کاملIn vitro determination of ruminal protein degradability using [15N]ammonia to correct for microbial nitrogen uptake.
An in vitro procedure was developed to estimate rate and extent of ruminal protein degradation using [15N]H3 to quantify uptake of protein degradation products for microbial protein synthesis. Incubations were conducted for 6 h in stirrer flasks with ruminal inoculum plus buffer, reducing solution, pectin, soluble carbohydrates, and added (15NH4)2SO4. Seven protein concentrates were tested in t...
متن کاملEffect of isolate of ruminal fibrolytic bacterial culture
Aim: The present study was conducted to evaluate the effect of bacterial culture supplementation on ruminal fibrolytic bacterial population as well as on survivability of inoculated bacterial strain in lactating Murrah buffaloes kept on high fibre diet. Materials and Methods: Fibrolytic bacterial strains were isolated from rumen liquor of fistulated Murrah buffaloes and live bacterial culture w...
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 2018
ISSN: 0021-8812,1525-3163
DOI: 10.1093/jas/sky251