New method for visualization of C-heterochromatin in synaptonemal complex spreads

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New method for visualization of C-heterochromatin in synaptonemal complex spreads

DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency ...

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ژورنال

عنوان ژورنال: Comparative Cytogenetics

سال: 2013

ISSN: 1993-078X,1993-0771

DOI: 10.3897/compcytogen.v7i2.5187