منابع مشابه
Improved β - Glucuroni - dase Reporter for Mammalian Gene Expression Analysis
1.Agatep, R., R.D. Kirkpatrick, D.L. Parchaliuk, R.A. Woods and R.D. Gietz. 1998. Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/ PEG) protocol. Technical Tips Online (http://tto.trends.com). 2.Boulton, S. and S. Jackson. 1996. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand b...
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Here we show that the transcriptional terminator element of human gastrin gene, which is the only element characterized to date in terms of its function in transcriptional termination, increases the transient expression levels of recombinant proteins. The expression of the beta-galactosidase gene was enhanced 3-4-fold in HeLa cells by inserting the terminator element of human gastrin gene at th...
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The Sos recruitment system (SRS) is a novel genetic method for detecting protein-protein interactions. The method is based on localizing Sos, a Ras guanyl nucleotide exchange factor (GEF), to the plasma membrane through interaction between two fusion proteins. Mammalian Ras can bypass the requirement for a functional Ras GEF and represents a predictable false positive in this system. This repor...
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the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...
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DNA probes for the studies of damaged strand excision during the nucleotide excision repair (NER) have been designed using the novel non-nucleosidic phosphoramidite reagents that contain N-[6-(9-antracenylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nAnt) and N-[6-(5(6)-fluoresceinylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nFlu) moieties. New lesion-imitating adducts being inserted into DNA s...
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ژورنال
عنوان ژورنال: Journal of Biological Sciences
سال: 2016
ISSN: 1727-3048
DOI: 10.3923/jbs.2016.178.187