New Amylase Inhibitor Present in Corn Seeds Active In Vitro Against Amylase from Fusarium verticillioides
نویسندگان
چکیده
منابع مشابه
A novel alpha-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds.
The major alpha-amylase inhibitor (AAI) present in the seeds of Amaranthus hypocondriacus, a variety of the Mexican crop plant amaranth, is a 32-residue-long polypeptide with three disulfide bridges. Purified AAI strongly inhibits the alpha-amylase activity of insect larvae (Tribolium castaneum and Prostephanus truncatus) and does not inhibit proteases and mammalian alpha-amylases. AAI was sequ...
متن کاملBiochemical Nature of a Natural α-Amylase Inhibitor from Wild Amaranth (Amaranthus paniculatus) Seeds
Endogenous á-amylase inhibitors exist widely in animals, plants and microorganisms. These inhibitors show remarkable structure variety with different modes of inhibition and specificity against different á-amylases. To explore the alpha-amylase inhibitors in wild amaranth, a novel proteinaceous inhibitor of á-amylase, named WAI-1, was purified and its structure and function were investigated in...
متن کاملCharacterization of the Α-amylase Inhibitor from the Seeds of Macrotyloma Uniflorum and Vigna Unguiculata
α-amylase inhibitors were purified and characterized from the seeds of Macrotyloma uniflorum (MUAI) and Vigna unguiculata (VUAI). MUAI was purified using CM-cellulose column while VUAI was purified using Poros HS-50 column followed by gel filtration on Sephadex G-75 column. The molecular weights of MUAI and VUAI as determined by gel filtration were 26.91 kD and 21.89 kD respectively. Both MUAI ...
متن کاملDetoxification of corn antimicrobial compounds as the basis for isolating Fusarium verticillioides and some other Fusarium species from corn.
The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively. Fusarium verticillioides (= F. moniliforme) is highly tolerant to M...
متن کاملΒ-Amylase from Starchless Seeds of Trigonella Foenum-Graecum and Its Localization in Germinating Seeds
Fenugreek (Trigonella foenum-graecum) seeds do not contain starch as carbohydrate reserve. Synthesis of starch is initiated after germination. A β-amylase from ungerminated fenugreek seeds was purified to apparent electrophoretic homogeneity. The enzyme was purified 210 fold with specific activity of 732.59 units/mg. Mr of the denatured enzyme as determined from SDS-PAGE was 58 kD while that of...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2003
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.2003.87.3.233