منابع مشابه
Separation of acid and neutral proteinases of brain.
1. Cerebral proteinases were separated on Sephadex G-100 columns into acid and neutral fractions free from cross-contamination. Acid proteinases were more stable and were purified by additional steps with salt and pH5.0 precipitations, column chromatography on DEAE- or CM-cellulose and free-flow electrophoresis. 2. The separation made it possible to study the properties of the partially purifie...
متن کاملRole of the Neutral Proteinases in Postmortem Muscle Protein Degradation and Meat Tenderness
Consumers consider tenderness to be the single most important component of meat quality. Recently, it has finally become clear that a lack of consistency in meat tenderness is one of the main problems facing the meat industry (Morgan et al., 1991b; Smith, 1992). A West coast supermarket chain (annual beef sales of $130 million) requests that its customers return any meat purchased, if they are ...
متن کاملCleavage of A-CAM by endogenous proteinases in cultured lens cells and in developing chick embryos.
We describe two truncated forms of A-CAM (N-cadherin) and present evidence suggesting that both forms are proteolytically derived from the intact A-CAM molecule. The first is a membrane-bound fragment of A-CAM displaying an apparent molecular weight of 78 kDa. This polypeptide, containing the C-terminal portion of the protein, may be generated in cultured chicken lens cells, either by a short t...
متن کاملPurification of neutral lens endopeptidase: close similarity to a neutral proteinase in pituitary.
A neutral endopeptidase (EC 3.4.24.5) that degrades alpha- and beta-crystallins occurs in mammalian lens. A procedure for purification of this enzyme from bovine lens is described. The enzyme appears to have a high molecular weight (Mr approximately equal to 700,000) and under denaturing conditions dissociates into at least eight polypeptide subunits with Mrs ranging from 24,000 to 32,000. A ne...
متن کاملCharacterization of neutral glycosphingolipids in human cataractous lens.
Neutral glycosphingolipids were purified from human senile cataractous lenses by a combination of solvent extraction, Folch's partition, acetylation, and column chromatography using DEAE-Sephadex and Iatrobeads. Six major glycosphingolipids (A-F) from monohexosylceramide to pentahexosylceramide were identified by sugar composition analysis, methylation analysis, secondary ion-mass spectrometry,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1962
ISSN: 0006-2936
DOI: 10.1042/bj0830274