Neurosteroids: oligodendrocyte mitochondria convert cholesterol to pregnenolone.
نویسندگان
چکیده
منابع مشابه
Neurosteroids: pregnenolone in human sciatic nerves.
The characterization and quantification of pregnenolone in human sciatic nerves were undertaken, following previous demonstration of the synthesis of this steroid in rat brain oligodendrocytes, to explore the hypothesis that Schwann cells may demonstrate the same biosynthetic activity. Pregnenolone was definitively identified by mass spectrometry and quantified by specific radioimmunoassay. Its...
متن کاملSterol carrier protein2. Delivery of cholesterol from adrenal lipid droplets to mitochondria for pregnenolone synthesis.
The ability of sterol carrier protein2 (SCP2) to mediate transfer of unesterified cholesterol from adrenal lipid inclusion droplets to mitochondria has been tested in an in vitro model system. Unlike mitochondrial utilization of cholesterol added in acetone or dimethyl sulfoxide, the unesterified cholesterol of lipid droplets did not provide a readily available source of substrate for mitochond...
متن کاملNeurosteroids: deficient cognitive performance in aged rats depends on low pregnenolone sulfate levels in the hippocampus.
Pregnenolone sulfate (PREG S) is synthesized in the nervous system and is a major neurosteroid in the rat brain. Its concentrations were measured in the hippocampus and other brain areas of single adult and aged (22-24 month-old) male Sprague-Dawley rats. Significantly lower levels were found in aged rats, although the values were widely scattered and reached, in about half the animals, the sam...
متن کاملAstrocytes and neurosteroids: metabolism of pregnenolone and dehydroepiandrosterone. Regulation by cell density
The rat central nervous system (CNS) has previously been shown to synthesize pregnenolone (PREG) and convert it to progesterone (PROG) and 7 alpha-hydroxy-PREG (7 alpha-OH PREG). Astrocytes, which participate to the regulation of the CNS function, might be involved in the metabolism of neurosteroids. Purified type 1 astrocytes were obtained from fetal rat forebrain with the use of selective cul...
متن کاملStudies on the Biosynthetic Conversion of Cholesterol into Pregnenolone
(20R)-20-f-Butyl-5-pregnene-3/3,20-diol, an analog of 20~ hydroxycholesterol, has been synthesized and its metabolism studied. Since C-22 in this synthetic compound is completely substituted, this position is unavailable for biological oxygenation. When injected intravenously into a rabbit, the t -butyl analog is metabolized to urinary pregnanediol. When incubated with sonicated mitochondria fr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.23.8215