Neuroprotective effects of tumor necrosis factor-α inhibitor on rat hippocampal organotypic slice cultures treated with the 1-42 <i>β</i>–amyloid

Background: Alzheimer disease (AD) is a chronic degenerative neuronal disease. Tumor necrosis factor (TNF) inhibitors involved in neuroinflammation AD pathogenesis can be potential treatment strategy for AD. Therefore, we performed organotypic hippocampal slice culture to investigate neuroprotective effects by TNF-α inhibitor from β-amyloid (Aβ)-induced neurotoxicity.Methods: We cultured the hippocampus of 7 days postnatal Sprague-Dawley rats with 450 µm thick slices. The tissue slices had been exposed 100 µM Aβ at an interval 2 since 14 vitro. Following co-treatment µg/ml adalimumab and Aβ, evaluated effect on induced neurotoxicity morphological observation cultures 1 propidium iodide (PI) measuring expression levels NeuN, Bcl2, tau proteins.Results: increase PI-positive fluorescence slice. as showed protective Aβ-induced neurotoxicity, reduced cell death CA1 region NeuN Bcl2 proteins was significantly decreased increased adalimumab. Phosphorylation protein adalimumab.Conclusions: Adalimumab culture, inhibited increasing phophorylated protein. These findings would useful baseline data field research development noble drugs neurodegenerative diseases.