Native Capillary Isoelectric Focusing for the Separation of Protein Complex Isoforms and Subcomplexes
نویسندگان
چکیده
منابع مشابه
Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes.
Here we report the use of capillary isoelectric focusing under native conditions for the separation of protein complex isoforms and subcomplexes. Using biologically relevant HIS-tag and FLAG-tag purified protein complexes, we demonstrate the separations of protein complex isoforms of the mammalian target of rapamycin complex (mTORC1 and 2) and the subcomplexes and different phosphorylation stat...
متن کاملCapillary Isoelectric Focusing
Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...
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A capillary isoelectric focusing (CIEF) method under denaturing condition has been developed for separation of a-keratins, highly cross-linked biomacromolecules, in horns of rhinoceros and buffalo. The a-keratins were denatured in 8 M urea and separated in the presence of ampholytes with applicable pH ranges of 3-7 or 3-10. In the preliminary results, it was demonstrated that the o~-keratins in...
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Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel electrophoresis and plays an important role in sample purification for proteomics. However, biases in protein size and pI resolution, as well as limitations in sample volume, gel capacity, sample loss, and experimental time, remain challenges. In order to address some of the limitations of traditional IEF, we present a mi...
متن کاملMicrofluidic Protein Separation by Free Flow Isoelectric Focusing
Disposable, inexpensive microfluidic devices have the potential to become a robust tool for proteomic research for separating and identifying proteins and protein complexes. In this work, a preparative scale free flow IEF isoelectric focusing (FFIEF) device was designed, investigated, and optimized. A constant electric field of up to 350V/cm was generated across the separation channel upon opti...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2010
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac101235k