Naked Eye Colorimetric Glucose Detection Using Microplate Reader

Diabetes in general can be classified as Type 1, 2, gestational diabetes mellitus that occur during pregnancy, and specific types of due to certain health condition. Monitoring glucose level is crucial order maintain a healthy lifestyle, especially among diabetic patients. Today, researchers looking for less painful, cheap, user-friendly noninvasive method monitor the body level. One promising approaches sweat based detection. In calibrate sensing device, we determined limit detection concentration liquid by well-established enzymatic reaction. This paper will discuss utilisation microplate reader measure sensitivity chromogenic change 50?L oxidase-peroxidase (GOD-POD) enzyme 1.67?L sample with different concentrations were mixed 96 well flat bottom microplate, absorbance detected using reader. The value was used calculate concentration. calculated compared known From experimental procedure, results showed consistency R2 = 0.9995