Naganishia cerealis IN1S2.5 oil production from the hydrolysate of NaOH-impregnated and catalyst steam explosion pretreated oil palm empty fruit bunch

NaOH-impregnation with catalyst steam explosion was found to be an efficient pretreatment method for oil palm empty fruit bunch (OPEFB) as a substrate production by Naganishia cerealis IN1S2.5. Cellulase hydrolysis of the pretreated OPEFB yielded glucose at 0.364 g/g. Investigation N. IN1S2.5 in hydrolysate revealed maximum yield (2.46 g/L) when C/P molar ratio adjusted 25.71, supplemented Ca2+ and Zn2+, set pH 4. The comprised oleic (37.6%), palmitic (36.2%), steric (17.9%) acids, all (w/w), major fatty acids. Predicted properties produced biodiesel indicated potential feedstock.