N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli.
نویسندگان
چکیده
منابع مشابه
Molecular structure of Escherichia coli PurT-encoded glycinamide ribonucleotide transformylase.
In Escherichia coli, the PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, catalyzes an alternative formylation of glycinamide ribonucleotide (GAR) in the de novo pathway for purine biosynthesis. On the basis of amino acid sequence analyses, it is known that the PurT transformylase belongs to the ATP-grasp superfamily of proteins. The common theme among members of ...
متن کاملOn the occurrence of glycinamide ribotide and its formyl derivative.
Inosinic acid is synthesized from small molecules such as glycine, formate, and CO2 by a multienzyme system present in pigeon liver (l-3). This paper reports the occurrence of two new aliphatic ribotides synthesized by this system and describes their isolation and metabolic interrelationships. These compounds have been shown to be precursors of inosinic acid (4-6) and have been assigned the fol...
متن کاملThe structure of glycinamide ribotide.
Materials-The pigeon liver extract preparation and the substrates required for the synthesis of glycinamide ribotide have been described previously (l-4). Intestinal phosphatase, purified grade, was obtained from Pentex, Inc., Kankakee, Illinois. Adenosine-5’-phosphate was obtained from the Ernst Bischoff Company, Inc., Ivoryton, Connecticut. Adenosine-3’-phosphate was obtained from the Schwarz...
متن کاملOn the mechanism of synthesis of glycinamide ribotide and its formyl derivative.
5-Phosphoribosylpyrophosphater was synthesized enzymatically and isolated by a slight modification of the procedure of Kornberg et al. (3, 4). PRPP used in preliminary experiments was generously supplied by Dr. A. Kornberg.2 Glycinamide was kindly provided by Dr. J. P. Greenstein. N-Formylglycine was synthesized by the method of Fischer and Warburg (5). Tetrahydrofolic acid was prepared accordi...
متن کاملThe Structure of Glycinamide Ribotide” by Richard
Materials-The pigeon liver extract preparation and the substrates required for the synthesis of glycinamide ribotide have been described previously (l-4). Intestinal phosphatase, purified grade, was obtained from Pentex, Inc., Kankakee, Illinois. Adenosine-5’-phosphate was obtained from the Ernst Bischoff Company, Inc., Ivoryton, Connecticut. Adenosine-3’-phosphate was obtained from the Schwarz...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34710-5