Mycoplasma spp. in captive snakes (Boa constrictor and Bothrops atrox) from Brazil

ABSTRACT: Although rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, order Squamata, three reports these microorganisms causing pythons have already been reported. This study aimed to evaluate occurrence Mycoplasma species captive snakes. A total 26 snakes families Pythonidae (13), Boidae (7), Viperidae (5) and Colubridae (1) from RioZoo, Brazil, were evaluated. Animals examined determine clinical signs consistent with any infectious disease. Tracheal swab samples collected Frey medium analyzed for presence spp.by isolation a genus-specific PCR. DNA sequencing analyses six positive by PCR carried out identify species. Using 19.23% (5/26) was positive, while 65.38% (17/26) animals Based on sequences obtained, there similarity spp. previously described phyton M. agassizii testudineum reported chelonians. is first report Viperidae. detected without signs. The resented identity (range, 95% 100%) others reptiles. There no relationship between