Mycoflora Associated with Barely Grains (Hordeum vulgare L.) in the Eastern Parts of Libya

During the harvest season (2019-2020), local variety of barley seeds (Hordeum vulgare L.) were collected from three different locations, Almerj, Gerdina, and Sultan; situated in eastern part Libya. The present experiment was performed to identify, compare natural mycoflora associated with among these also evaluate sodium hypochlorite’s application, as seed disinfectants against fungal contaminators. Barley surface disinfected a 4% hypochlorite for two minutes or washed only deionized water (control) before plating on potato dextrose agar (PDA) medium. following parameters recorded calculated: survival (G %), frequency (F), isolation (IF) relative abundance (RD). Four species identified Aspergillus niger van Tiegh, A. flavus Link (ascomycetes), Rhizopus stolonifera (Ehrenb. :Fr.) Vuill (zygomycetes), Bipolaris australiensis (Bugnicourt) (ascomycetes). most predominant recovered followed by B. australiensis, R. stolonifera, flavus, (18.5%), (9.67%) (2.84%), (4.65%), respectively. Results showed Sultan had highest germination, Almerj 64.5 %, 44.5%, 20.5 Moreover, seed’s pretreatment origin no significant effect fungi.