منابع مشابه
MutL traps MutS at a DNA mismatch.
DNA mismatch repair (MMR) identifies and corrects errors made during replication. In all organisms except those expressing MutH, interactions between a DNA mismatch, MutS, MutL, and the replication processivity factor (β-clamp or PCNA) activate the latent MutL endonuclease to nick the error-containing daughter strand. This nick provides an entry point for downstream repair proteins. Despite the...
متن کاملMutation detection with MutH, MutL, and MutS mismatch repair proteins.
Escherichia coli methyl-directed mismatch repair is initiated by MutS-, MutL-, and ATP-dependent activation of MutH endonuclease, which cleaves at d(GATC) sites in the vicinity of a mismatch. This reaction provides an efficient method for detection of mismatches in heteroduplexes produced by hybridization of genetically distinct sequences after PCR amplification. Multiple examples of transition...
متن کاملMutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA
To avoid mutations in the genome, DNA replication is generally followed by DNA mismatch repair (MMR). MMR starts when a MutS homolog recognizes a mismatch and undergoes an ATP-dependent transformation to an elusive sliding clamp state. How this transient state promotes MutL homolog recruitment and activation of repair is unclear. Here we present a crystal structure of the MutS/MutL complex usin...
متن کاملmutS, mutL and mutH genes. A homodimer of MutS binds to mismatches in DNA and a homodimer of MutL coordinates mismatch recognition
sequences (recently reviewed in Buermeyer et al., 1999; Kolodner and Marsischky, 1999; Harfe and Jinks-Robertson, 2000; Hsieh, 2001). In the most thoroughly characterized Escherichia coli MMR system, which serves as a paradigm for eukaryotic MMR studies, initiation of MMR requires the products of mutS, mutL and mutH genes. A homodimer of MutS binds to mismatches in DNA and a homodimer of MutL c...
متن کاملBiochemical Properties of MutL, a DNA Mismatch Repair Endonuclease
DNA mismatch repair (MMR) is one of the most widely conserved DNA repair systems, which repairs mismatched bases generated mainly by the error of DNA polymerases during replication (Friedberg, et al., 2006, Iyer, et al., 2006, Kunkel, et al., 2005, Morita, et al., 2010). MMR increases the replication fidelity by 20 to 400-fold (Schaaper, 1993). Mutations and epigenetic silencing in MMR genes ca...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2015
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1505655112