Mutations across Murine Hepatitis Virus nsp4 Alter Virus Fitness and Membrane Modifications

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Mutations across murine hepatitis virus nsp4 alter virus fitness and membrane modifications.

UNLABELLED A common feature of infection by positive-sense RNA virus is the modification of host cell cytoplasmic membranes that serve as sites of viral RNA synthesis. Coronaviruses induce double-membrane vesicles (DMVs), but the role of DMVs in replication and virus fitness remains unclear. Coronaviruses encode 16 nonstructural proteins (nsps), three of which, nsp3, nsp4, and nsp6, are necessa...

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Genetic analysis of Murine hepatitis virus nsp4 in virus replication.

Coronavirus replicase polyproteins are translated from the genomic positive-strand RNA and are proteolytically processed by three viral proteases to yield 16 mature nonstructural proteins (nsp1 to nsp16). nsp4 contains four predicted transmembrane-spanning regions (TM1, -2, -3, and -4), demonstrates characteristics of an integral membrane protein, and is thought to be essential for the formatio...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 2014

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.02776-14