Mutational and crystallographic analysis ofl-amino acid oxidase/monooxygenase fromPseudomonassp. AIU 813: Interconversion between oxidase and monooxygenase activities

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Mutational and crystallographic analysis of l-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities

In this study, it was shown for the first time that l-amino acid oxidase of Pseudomonas sp. AIU813, renamed as l-amino acid oxidase/monooxygenase (l-AAO/MOG), exhibits l-lysine 2-monooxygenase as well as oxidase activity. l-Lysine oxidase activity of l-AAO/MOG was increased in a p-chloromercuribenzoate (p-CMB) concentration-dependent manner to a final level that was five fold higher than that o...

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Ligand complex structures of l‐amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 and its conformational change

l-Amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 (l-AAO/MOG) catalyzes both the oxidative deamination and oxidative decarboxylation of the α-group of l-Lys to produce a keto acid and amide, respectively. l-AAO/MOG exhibits limited specificity for l-amino acid substrates with a basic side chain. We previously determined its ligand-free crystal structure and identified a key residu...

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15 صفحه اول

D-Amino Acid Oxidase and Metagenomics

D-amino acid oxidase (DAAO, EC 1. 4. 3. 3) converts D-amino acid to a corresponding α-keto acid via deamination. D-amino acid oxidase is one of the most important enzymes responsible for maintenance proper level of D-amino acids, which play a key role in regulation of many processes in living cells. This paper summary the applications of D-amino acid oxidase in agricultural and industry practic...

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ژورنال

عنوان ژورنال: FEBS Open Bio

سال: 2014

ISSN: 2211-5463

DOI: 10.1016/j.fob.2014.02.002