Mutagenesis using Tn917 in Bacillus licheniformis
نویسندگان
چکیده
منابع مشابه
Chromosomal insertions of Tn917 in Bacillus subtilis.
We describe 46 insertions of the Streptococcus faecalis transposon Tn917 into the chromosome of Bacillus subtilis. These insertion mutations were mapped genetically. Some caused auxotrophic requirements, and others were cryptic. These insertions were scattered around the B. subtilis chromosome. The mutant strains were useful in several ways for mapping and cloning B. subtilis genes and were add...
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Transposon Tn9l7 from Steptococcus faecalis (12) is presently the most useful transposon for Bacillus subtilis genetic manipulation. It is 5.3 kilobases in length, confers inducible erythromycin resistance, and has terminal inverted repeats very closely related to the Tn3 family of transposons of gram-negative organisms (11). Youngman et al. (16) constructed a Tn917-carrying plasmid, pTV1, that...
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The present study is concerned with the improvement of Bacillus licheniformis strain GCB30 for alpha amylase production in 250 ml Erlenmeyer flasks. Chemical mutation using ethyl methanesulfonate (EMS 50-300 μ/ml) was undertaken for 10-60 min. Twenty eight isolates were selected on the basis of clear zones of starch hydrolysis. Only one isolate designated as B. licheniformis EMS-200 gave 102.78...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1990
ISSN: 0378-1097
DOI: 10.1016/0378-1097(90)90058-x