Murine intracisternal type A particles: a biochemical characterization
نویسندگان
چکیده
منابع مشابه
Structural organization of murine intracisternal A particles.
Isolated murine intracisternal A particles have a distinctive set of structural properties as compared to the recognized oncogenic ribonucleic acid tumor viruses. A particles consist of two closely approximated concentric shells. The outer shell is largely, but not entirely, susceptible to treatment with detergents such as deoxycholate and Triton X-100. The inner shell is resistant to these age...
متن کاملCharacterization of an endogenous RNA-dependent DNA polymerase associated with murine intracisternal A particles.
An RNA-dependent DNA polymerase associated with intracisternal A particles has been characterized. The enzyme required Mg(2+) or Mn(2+), dithiothreitol and the presence of all four deoxyribonucleoside triphosphates for the expression of maximal activity. Sensitivity of the endogenous RNA-dependent DNA polymerase activity to a low concentration of pancreatic ribonuclease in the presence of a hig...
متن کاملMurine intracisternal A type particles fail to separate from the membrane of the endoplasmic reticulum.
Analysis of serial sections of murine cells containing intracisternal A particles revealed that over 99% of all A particles remain in a budding configuration. This indicates that these particles fail to detach from the membrane of the endoplasmic reticulum. This observation explains how, despite their intracellular abundance in certain murine tumors, no extracellular A-type particles can be found.
متن کاملRNA associated with murine intracisternal type A particles codes for the main particle protein.
Intracisternal type A particles were isolated from MOPC-104E myeloma grown subcutaneously and from N 4 neuroblastoma cells in culture. Polyadenylated RNA was prepared from the particles and tested in a cell-free translation system derived from rabbit reticulocytes. RNA from the two sources directed the synthesis of multiple polypeptides with similar distributions of electrophoretic mobilities i...
متن کاملIntracisternal type A particles occurring in foreign body-induced sarcomas.
Eight sarcomas induced in mouse strains CBA/H, CBA/HT6, and C57BL/10ScSn (or their hybrids) by s.c. implantation of smooth or roughened plastic films or glass pieces were examined electron microscopically. Type A virus particles were detected within dilated ergastoplasmic cisternae of the sarcoma cells from five mice. The doughnut-shaped particles measured 90 to 100 nm and were seen to bud from...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1975
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.16.4.887-896.1975