Multiscale Characterization of Engineered Cardiac Tissue Architecture
نویسندگان
چکیده
منابع مشابه
Myofibrillar architecture in engineered cardiac myocytes.
Morphogenesis is often considered a function of transcriptional synchrony and the spatial limits of diffusing mitogens; however, physical constrainment by the cell microenvironment represents an additional mechanism for regulating self-assembly of subcellular structures. We asked whether myocyte shape is a distinct signal that potentiates the organization of myofibrillar arrays in cardiac muscl...
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We developed a high-throughput screening assay that allows for relative comparison of the twitch force of millimeter-scale gel-based cardiac tissues. This assay is based on principles taken from traction force microscopy and uses fluorescent microspheres embedded in a soft polydimethylsiloxane (PDMS) substrate. A gel-forming cell suspension is simply pipetted onto the PDMS to form hemispherical...
متن کاملControlling the contractile strength of engineered cardiac muscle by hierarchal tissue architecture.
The heart is a muscular organ with a wrapping, laminar structure embedded with neural and vascular networks, collagen fibrils, fibroblasts, and cardiac myocytes that facilitate contraction. We hypothesized that these non-muscle components may have functional benefit, serving as important structural alignment cues in inter- and intra-cellular organization of cardiac myocytes. Previous studies ha...
متن کاملCardiac grafting of engineered heart tissue in syngenic rats.
BACKGROUND Cell grafting has emerged as a novel approach to treat heart diseases refractory to conventional therapy. We hypothesize that survival and functional and electrical integration of grafts may be improved by engineering cardiac tissue constructs in vitro before grafting. METHODS AND RESULTS Engineered heart tissue (EHT) was reconstituted by mixing cardiac myocytes from neonatal Fisch...
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Take-home messages: • A broad range of cardiovascular conditions affect the myocardium. • The presence and extent to which the myocardium is affected can be estimated with magnetic resonance-based tissue characterization techniques. • The extent to which these techniques have been validated varies; the certainty with which a cardiac magnetic resonance (CMR)-based tissue characteristic is assign...
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ژورنال
عنوان ژورنال: Journal of Biomechanical Engineering
سال: 2016
ISSN: 0148-0731,1528-8951
DOI: 10.1115/1.4034656