Multiresidue analysis of 184 pesticides in high-fat fish feed using a new generic extraction method coupled with gas and liquid chromatography-tandem mass spectrometry

Abstract Animal feed is typically plant-based and can contain pesticide residues. Methods for testing food samples, such as the Quick Easy Cheap Effective Rugged Safe (QuEChERS) method or Swedish Ethyl Acetate (SweEt) method, successfully extract many However, nonpolar pesticides, organochlorine show poor recovery when extracted from lipid-rich samples. The previously developed water-acetonitrile-heptane-solid-phase-extraction (WAHSPE) shows better recoveries pesticides but requires two injections per sample instrument. Here, we present a modified version of WAHSPE in fish using one injection Of 184 tested, 179 met European Union Legislation’s validation criteria at spike level 50 μg/kg, showing between 70 120% relative standard deviation (RSD) below 20%. Organochlorine accounted 14 tested compounds.