Multiplex polymerase chain reaction for screening avian pathogenicEscherichia colifor virulence genes

Characterizing avian Escherichia coli isolates with multiplex polymerase chain reaction.

Colibacillosis caused by Escherichia coli infections account for significant morbidity and mortality in the poultry industry. Yet, despite the importance of colibacillosis, much about the virulence mechanisms employed by avian E. coli remains unknown. In recent years several genes have been linked to avian E. coli virulence, many of which reside on a large transmissible plasmid. In the present ...

Rfbo157 by Multiplex Polymerase Chain Reaction

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.

Development of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus

Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...

Detection of three avian respiratory viruses by single-tube multiplex reverse transcription-polymerase chain reaction assay.

Acute respiratory tract infections are leading causes of morbidity in poultry farms throughout the world. Avian pneumovirus (APV), avian influenza virus (AIV), and Newcastle disease virus (NDV) have been recognized as the most important pathogens of both chicken and turkeys. Single-virus reverse transcription-polymerase chain reaction (sRT-PCR) assays are used extensively to detect these viruse...