Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing
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چکیده
منابع مشابه
Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing
Next-generation sequencing (NGS) technologies have enabled genome re-sequencing for exploring genome-wide polymorphisms among individuals, as well as targeted re-sequencing for the rapid and simultaneous detection of polymorphisms in genes associated with various biological functions. Therefore, a simple and robust method for targeted re-sequencing should facilitate genotyping in a wide range o...
متن کاملTargeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
Whole transcriptome RNA-sequencing is a powerful tool, but is costly and yields complex data sets that limit its utility in molecular diagnostic testing. A targeted quantitative RNA-sequencing method that is reproducible and reduces the number of sequencing reads required to measure transcripts over the full range of expression would be better suited to diagnostic testing. Toward this goal, we ...
متن کاملSESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing
SUMMARY Characterizing genetic diversity through genotyping short amplicons is central to evolutionary biology. Next-generation sequencing (NGS) technologies changed the scale at which these type of data are acquired. SESAME is a web application package that assists genotyping of multiplexed individuals for several markers based on NGS amplicon sequencing. It automatically assigns reads to loci...
متن کاملRunning Head: HIGHLY MULTIPLEXED AMPLICON-BASED PHYLOGENOMICS 1 Title: HiMAP: robust Phylogenomics from Highly Multiplexed Amplicon sequencing
15 High-throughput sequencing has fundamentally changed how molecular phylogenetic datasets 16 are assembled, and phylogenomic datasets commonly contain 50-100-fold more loci than those 17 generated using traditional Sanger-based approaches. Here, we demonstrate a new approach for 18 building phylogenomic datasets using single tube, highly multiplexed amplicon sequencing, 19 which we name HiMAP...
متن کاملCorrigendum: A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes
[This corrects the article on p. 731 in vol. 6, PMID: 26236305.].
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2018
ISSN: 1664-462X
DOI: 10.3389/fpls.2018.00201