Multiplex Genome Editing in Chinese Hamster Ovary Cell Line Using All-in-One and HITI CRISPR Technology
نویسندگان
چکیده
منابع مشابه
Propranolol induced chromosomal aberrations in Chinese hamster ovary cell line
Propranolol (PL), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. The present study was concerned with assessing the cytogenetic effects of this drug on Chinese hamster ovary (CHO) cell line. MTT assay was then carried out to determine the cytotoxicity index (IC50) of the drug. The IC50 value of PL was 0.43±0.02 mM. To investigate the clastogenic effect...
متن کاملpropranolol induced chromosomal aberrations in chinese hamster ovary cell line
propranolol (pl), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. the present study was concerned with assessing the cytogenetic effects of this drug on chinese hamster ovary (cho) cell line. mtt assay was then carried out to determine the cytotoxicity index (ic50) of the drug. the ic50 value of pl was 0.43±0.02 mm. to investigate the clastogenic effect...
متن کاملMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. ...
متن کاملcloning and expression of luteinizing hormone subunits in chinese hamster ovary cell line
background: luteinizing hormone (lh) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. the application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively. m aterials and methods: in the present study the alpha and beta subunits of human lh gene were cloned into the pegfp-n1 expression vector and produc...
متن کاملEditing of the MALAT1 Gene in MDA-MB-361 Breast Cancer Cell Line using the Novel CRISPR Method
Introduction: Long non-coding RNAs play an important role in regulating gene expression, RNA processing, histone modification, and rearrangement of chromatin genes. These molecules can also be involved in many biological processes, such as organogenesis, cell differentiation, development, genome imprinting, quantitative compensation, and tumorigenesis. High expression of MALAT1 (a type of lncRN...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Advanced Pharmaceutical Bulletin
سال: 2020
ISSN: 2228-5881,2251-7308
DOI: 10.34172/apb.2021.032