Multiple RFLPs of human complement component nine (C9) detected by Taql
نویسندگان
چکیده
منابع مشابه
TaqL and MspL RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA.
Source/Description: The XMEL4 clone is a 1.5 kb cDNA subcloned into the EcoRI site of pBluescript SKII +. XMEIA was isolated from a human melanoma cDNA library. Sequence analysis showed complete homology between XMEL4 and the 2,3-biphosphoglycerate mutase (BPGM) cDNA sequence (1, 2). Polymorphism: Both Taql and Mspl detect two-allele polymorphisms when probed with XMEIA Frequency: Allele freque...
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Using specific immune sera, C7, C9, and C3 activator were detected in sera from human fetuses more than 16 weeks old and in newborn samples. Levels of C9 in cord sera ranged between 10 and 30% of those present in sera from adult subjects. The mean value of Ce activator was about half that in maternal blood. The mean level of C7 in newborns was nearly 70% of the amount in normal adults.
متن کاملFirst Component of Human Complement
The active esterase hydrolyzed certain synthetic amino acid esters, among which N-acetyl-L-tyrosine ethyl ester was most susceptible (3-5). This substrate was hydrolyzedmaximally between pH 7.5 and 8.2 and at 41°C. The esterase was inhibited by a heat-labile factor in human serum which did not appear to be related to any of the known components of complement, and by certain sulfbydryl-containin...
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The membrane attack complex (MAC)/perforin-like protein complement component 9 (C9) is the major component of the MAC, a multi-protein complex that forms pores in the membrane of target pathogens. In contrast to homologous proteins such as perforin and the cholesterol-dependent cytolysins (CDCs), all of which require the membrane for oligomerisation, C9 assembles directly onto the nascent MAC f...
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The human complement C9 protein (∼65 kDa) is a member of the complement pathway. It plays an essential role in the membrane attack complex (MAC), which forms a lethal pore on the cellular surface of pathogenic bacteria. Here, we charted in detail the structural microheterogeneity of C9 purified from human blood serum, using an integrative workflow combining high-resolution native mass spectrome...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.10.3112-a