منابع مشابه
Multiphoton-Polymerized 3D Protein Assay
Multiphoton polymerization (MPP) enables 3D fabrication of micro- and nanoscale devices with complex geometries. Using MPP, we create a 3D platform for protein assays. Elevating the protein-binding sites above the substrate surface allows an optically sectioned readout, minimizing the inevitable background signal from nonspecific protein adsorption at the substrate surface. Two fluorescence-lin...
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We report for the first time on the redox multiphoton polymerization of an organic-inorganic composite material, in which one of the components, a vanadium metallo-organic complex, initiates the polymerization. The composite employs multiphoton absorption to self-generate radicals by photoinduced reduction of the metal species from vanadium (V) to vanadium (IV). We exploit this material for the...
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Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette. No commercial kit was available for Bradford microplate assay in Iran. Meanwhile, imported Bradford commercial kits are very expensive and have a long delivery time in Iran. Till now no study or document on Br...
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We demonstrate the applicability of time-correlated single photon counting multiphoton microscopy to the spatiotemporal localisation of protein-protein interactions in situ. An Example of a new fluorescent protein variant with enhanced properties are given and the development of a FRET biosensor for simultaneous measurement of multiple intraand inter-molecular interactions is illustrated by exp...
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ژورنال
عنوان ژورنال: ACS Applied Materials & Interfaces
سال: 2018
ISSN: 1944-8244,1944-8252
DOI: 10.1021/acsami.7b13183