Multidrug-resistant Bacterial Isolates in Bovine Subclinical Mastitis from Southern Rajasthan

Background: In the present study two hundred milk samples were collected from cows and buffaloes with no history of clinical mastitis in ongoing lactation, Sirohi district Southern Rajasthan. Methods: The pooled sample (collected each quarter) examined for status subclinical by Modified California test Somatic cell count respectively. Positive further investigated isolation identification major mastitis-causing pathogens: S. aureus, predominant Streptococcal species E. coli assessing antimicrobial resistance models southern Result: results current indicate high levels multi-drug antibiotic among bacteria that commonly cause mastitis, particularly ampicillin, penicillin, tetracycline, erythromycin methicillin. However, highest sensitivity was conferred to ceftriaxone, gentamicin, co-trimoxazole, suggestive judicious use these antibiotics treatment bovine mastitis. Concurrent implementation gradient PCR indicated presence mecA blaZ genes 51.9% 81.4% aureus isolates, Meanwhile, 56.6% streptococcal isolate contained tetracycline-conferring tetM gene none streptococci ermB gene. 92.3% isolates tetA tetB tetracycline resistance.