Multi-omics approach for comparative studies of monoclonal antibody producing CHO cells
نویسندگان
چکیده
منابع مشابه
Multi-omics approach for comparative studies of monoclonal antibody producing CHO cells
Background Monoclonal antibody (mAb) therapy has revolutionized the treatment of a vast range of diseases, mostly in the areas of oncology and autoimmune/inflammatory disorders [1]. With a world market exceeding 60 billion USD per year and six mAb related products in the top 10 selling drugs, the industry continues to grow at a fast rate [2]. Chinese hamster ovary (CHO) cells are the preferred ...
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A study of the kinetics of antibody-producing cells has been carried out by the use of rosette formation for detection of individual antibody-producing cells, and labeling with tritiated thymidine, in cells obtained from mouse spleens at intervals after injection of SRBC. Following a primary injection of the antigen, the number of RFC per million cells was found to increase to a peak at 5 days,...
متن کاملAnalyzing Clonal Variation of Monoclonal Antibody-Producing CHO Cell Lines Using an In Silico Metabolomic Platform
Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins. In this work, we have studied the distinct metabolism of CHO cell clones harboring an efficient inducible expression system, based on the cumate gene switch, and displaying different expression levels, high and low productiv...
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Production instability currently limits the use of mammalian cells for industrial production of therapeutic proteins. We have previously reported that the loss of productivity in recombinant monoclonal antibody producing Chinese Hamster Ovary (CHO-mAb) cell lines is mainly due to a decrease in heavy chain (HC) and light chain (LC) transcripts. Molecular analysis indicates that the decreased mRN...
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Background The large-scale production of biopharmaceuticals commonly relies on the use of suspension cell cultures, since they provide higher yields than adherent cultures. However, most mammalian cells grow in adherent mode and therefore need to go through a process of adaptation to suspended growth, which is not always simple or feasible. In this context, microcarrier cultures have introduced...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2015
ISSN: 1753-6561
DOI: 10.1186/1753-6561-9-s9-o8