MS-IRS PCR: A Simple Method for the Isolation of Microsatellites

MS-IRS PCR: a simple method for the isolation of microsatellites.

Microsatellites (MS) are excellent genetic markers because of their prevalence in eukaryotic genomes, ease of typing by PCR and high degree of polymorphism. MS-based genetic maps of the human and mouse genomes have been constructed with average intermarker interval sizes of 1.6 cM and 0.42 cM, respectively (1,6). While these maps are essential resources for gene mapping, their resolution may no...

A simple method for the determination of serum cholesterol

investigating the feasibility of a proposed model for geometric design of deployable arch structures

deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...

A simple PCR procedure for discovering microsatellites from small insert libraries

Microsatellite discovery from genomic libraries is tedious because of the low number of clones that contain inserts and costly because of screening methodologies. A new procedure for screening clones for microsatellite DNA is described herein. Instead of colony hybridization, a polymerase chain reaction (PCR) with two vector standard primers and one synthesized repeat primer was used to directl...

LC-MS/MS: a rapid and simple new method for the determination of carbapenem β-lactamases.

We investigated the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for pan-drug-resistant Acinetobacter baumannii (PDR-AB) with carbapenem resistance. Eight strains were randomly selected from 84 clinical isolates of PDR-AB strains obtained by the Kirby-Bauer and agar dilution methods. An efflux pump inhibition test was used to screen for the efflux pump phenotype. An ...