MOUSE ISOANTIGENS: SEPARATION OF SOLUBLE TL (THYMUS-LEUKEMIA) ANTIGEN FROM SOLUBLE H-2 HISTOCOMPATIBILITY ANTIGEN BY COLUMN CHROMATOGRAPHY

Mouse Isoantigens: Separation of Soluble Tl (thymus-leukemia) Antigen from Soluble H-2 Histocompatibility Antigen by Column Chromatography

Mouse H-2 histocompatibility antigen has been extracted, solubilized, and partly purified from the cells of an A strain spontaneous leukemia carrying TL (thymus-leukemia) antigens. H-2 and TL. 1, 2, 3 activities were measured by inhibition of the cytotoxic effect of the corresponding isoantibodies. TL activity was associated with the H-2 active fraction obtained by solubilization and fractionat...

MOUSE ISOANTIGENS: SEPARATION OF SOLUBLE TL (THYMUS- LEUKEMIA) ANTIGEN FROM SOLUBLE H-2 HISTOCOMPATI- BILITY ANTIGEN BY COLUMN CHROMATOGRAPHY* Bx

Serological study of cell membrane antigens in the mouse has revealed several isoanfigenic systems demonstrable by cytotoxic isoantisera. Four of these systems of antigens are exclusively or predominantly represented on thymocytes. One of these, TL (thymus-leukemia) (1), is exclusively represented on thymocytes: two (Ly-A and Ly-B) have predominant representation on thymocytes and are present a...

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

ANTIGENIC MODULATION IN VITRO I. Fate of Thymus-Leukemia (TL) Antigen-Antibody Complexes Following Modulation of TL Antigenicity from the Surfaces of Mouse Leukemia Cells and Thymocytes*

Cell surface antigens of the thymus-leukemia (TL) 1 system in the mouse are detectable only on thymocytes and cells of certain leukemias {1-3). Although TL antigens are not expressed on thymocytes of all mouse strains, leukemias developing in T L strains may be phenotypically TL+, suggesting that TL structural genes may be present in all mouse strains, and possibly in all cells (2). The appeara...

Expression of the thymus leukemia antigen in mouse intestinal epithelium.

The Qa and Tla regions of the mouse major histocompatibility complex contain a series of genes encoding proteins with structural similarity to the class I transplantation antigens of the same complex. In contrast to the genes encoding the transplantation antigens, the Qa and Tla genes show very little polymorphism. Function(s) of the proteins encoded by the Qa and Tla loci remain an enigma. Rec...