Morphometric parameters and photosynthetic performance of in vitro propagated pineapple

Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family high socioeconomic interest, widely cultivated around world. The multiplication pineapple seedlings in field can be time-consuming, requiring significant labor investment. objective this study was to evaluate performance continuous and temporary immersion systems micropropagation scale-up species. Shoots were obtained from explants subcultured flasks with gelled culture medium without gas exchange. shoots transferred liquid MS supplemented 2mM NAA, 4mM BAP, four different devices: sealed flasks, semipermeable membranes, RITA®, twin-flasks. After 45 days cultivation, plant growth, fresh mass increment, stomatal density abaxial surface leaves, maximum quantum yield photosystem II, contents chlorophyll carotenoids analyzed. Significant differences observed density, carotenoids. twin-flasks RITA® devices revealed better results morphological parameters, such as growth while treatments membrane stood out content.