Morpho-taxometrical and molecular characterization of Steinernema abbasi (Nematoda: Steinernematidae) and its pathogenicity and generative potential against lepidopteran pests

Abstract Background An entomopathogenic nematode (EPN) was recovered by using Galleria baiting technique from the soils of marigold fields Noida, Uttar Pradesh, India. Based on morphological, morphometrical and molecular characterizations, isolated strain identified as Steinernema abbasi tagged CS38. The conspecific to original description with minor deviations. Infective juveniles (IJs) present were longer than description. Results Molecular analysis done ITS1-5.8S-ITS2 D2D3 regions. Pathogenicity generative potential CS38 tested against larvae 3 lepidopteran insect pests, namely, mellonella L., Helicoverpa armigera (Hb.) Spodoptera litura (Fab.). Different concentrations IJs/larva, viz . 25, 50, 100, 200 used for bioassay trails. All experiments repeated thrice reach optimum authenticity. bioassays revealed that isolate highly virulent pests caused (100%) mortality within 48 h under laboratory conditions. Generative studied S. recorded high in G. (19 × 10 4 IJs/larva) at 100 IJs/larva concentration, followed H. (63.4 (60.5 IJs/larva). Conclusions Isolate is an indigenous, dominant can be utilized a biological control agent three insects. Moreover, it commercialization production EPN-based biopesticide added Integrated Pest Management Indian agriculture system.