Morpho-Physiological and Transcriptome Analyses Provide Insights into the Wizened Bud Formation in Pear Trees

Wizened buds are frequently observed in pear (Pyrus spp.) trees, which greatly reduces the yield. However, little is known about mechanism of wizened bud formation. Here, we analyzed physiological and transcriptomic differences between normal ‘710’ trees. The results indicated that sorbitol boron (B) contents, during differentiation, were significantly reduced buds. microscopic observation transcriptome analysis revealed collapse organ structure cell wall loosening process may have a close relation with Moreover, transcript levels PpyMYB39.1 its downstream genes (PpyHT1, PpyHT2, PpyPMEI1 PpyPMEI2) found pentose glucuronate interconversion pathway (PpyPME3, PpyPL18.1, PpyPL18.2, PpyPG1 PpyPG2) concentration pectin-degradation-related enzymes increased Correspondingly, pectin was Taken together, promote degradation decrease carbohydrate transport by regulating supposed to play vital role formation resulting from process. Our study provides fundamental insights into strategies reduce occurrence