More Than 40,000 Transcripts, Including Novel and Noncoding Transcripts, in Mouse Embryonic Stem Cells
نویسندگان
چکیده
منابع مشابه
Noncoding RNA transcripts.
Recent analyses of the human genome and available data about the other higher eukaryotic genomes have revealed that, in contrast to Eubacteria and Archaea, only a small fraction of the genetic material (ca 1.5%) codes for proteins. Most of genomic DNA and its RNA transcripts are involved in regulation of gene expression, which can be exerted at either the transcriptional level, controlling whet...
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Large-scale studies of mammalian genome transcription reveal that a large proportion of the genome is transcribed. It remains an open question whether the identified transcripts are functional. Here, we searched for computational evidence to support the functionality of 34,030 noncoding RNA (ncRNA) transcripts reported by the Fantom3 project. We show that compared to control sets, the Fantom nc...
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Non-protein-coding RNAs (ncRNAs) are increasingly being recognized as having important regulatory roles. Although much recent attention has focused on tiny 22- to 25-nucleotide microRNAs, several functional ncRNAs are orders of magnitude larger in size. Examples of such macro ncRNAs include Xist and Air, which in mouse are 18 and 108 kilobases (Kb), respectively. We surveyed the 102,801 FANTOM3...
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purpose: The aim of the present study is to understand if EBs can generate neural rosette upon co-culture with chick embryo somites. Materials and Methods: The mouse ES cells, line Royan Bl, were cultured in hanging drops to induce embryoid bodies (EBs) formation. Somites were isolated from the chick embryos and then embedded in alginate solution. Finally, alginate beads containing somites were...
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ژورنال
عنوان ژورنال: Stem Cells
سال: 2006
ISSN: 1066-5099,1549-4918
DOI: 10.1634/stemcells.2006-0005