Mononucleotide repeat polymorphism in the APP gene

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

Ligase-based detection of mononucleotide repeat sequences.

Up to 15% of all colorectal cancers are considered to be replication error positive (RER(+)) and contain mutations at hundreds of thousands of microsatellite repeat sequences. Recently, a number of intragenic mononucleotide repeat sequences have been demonstrated to be targets for inactivating genes in RER(+)colorectal tumors. In this study, thermostable DNA ligases were tested for the ability ...

Dinucleotide repeat polymorphism in the human angiotensinogen gene.

PCR Conditions: PCR was performed in a total volume of 20 microliters with 200 ng of genomic DNA, 8 pmoles of each primer, 2 mM MgCl2, 200 mM dNTP, 50 mM KC1, 5 mM Tris-HCl, pH 8.3, 0.01% gelatin, 0.5 U Taq polymerase (Boehringer). Primer Ang5 was 5' end labeled with P 7-ATP with the resulting specific activity 0.1-0.05 /iCi/pmol. Amplification was for 35 cycles with denaturation at 94°C 1 min,...

Tri- and tetranucleotide repeat polymorphism in the LIPA gene.

Short mononucleotide repeat sequence variability in mismatch repair-deficient cancers.

Mismatch repair-deficient cancers are characterized by widespread insertions and deletions in microsatellite sequences, including those comprised of mononucleotide repeats. Such alterations have been observed in relatively short mononucleotide tracts in several genes and often are interpreted to indicate that the affected genes normally act as tumor suppressors. To aid in the interpretation of ...