Monoclonal antibodies to porcine factor VIII coagulant and their use in the isolation of active coagulant protein
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منابع مشابه
Monoclonal antibodies to porcine factor VIII coagulant and their use in the isolation of active coagulant protein.
Partially purified preparations of porcine factor VIII:C were used to immunize mice and spleen cells from the immunized animals were fused to NS-1 mouse myeloma cells. The ability of hybrid culture fluids to bind factor VIII:C was detected with a radiolabelled, affinity-purified, human antihuman VIII:C inhibitor. Three cloned hybrid lines have been obtained that preferentially bind to VIII:C wh...
متن کاملHeterogeneity of autoantibodies to factor VIII: differences in specificity for apparently distinct antigenic determinants of factor VIII coagulant protein.
The interference of antibodies to factor VIII coagulant protein (VIII:C) of 9 nonhemophilic patients with the binding to factor VIII coagulant antigen (VIII:CAg) of a reference hemophilic 125I-Fab' reagent, used in a liquid phase VIII:CAg assay, was studied. The binding competition was estimated from immunoradiometric assay (IRMA) dose-response slope of VIII:CAg present in patient plasma, inter...
متن کاملInactivation of factor VIII coagulant activity by two different types of human antibodies.
Human antibodies that inactivate factor VIII procoagulant activity (VIII:C) are heterogeneous in their kinetic properties. We report there here the properties of four type I and four type II antibodies classified according to Biggs et al. Type I antibodies have second-order inactivation kinetics and completely destroy VIII:C when present in high concentration; type II antibodies have more compl...
متن کاملFactor Viii Coagulant Activity and Factor Viii-like Antigen: Independent Molecular Entities
Factor VIII coagulant activity (VIII(C)) and the von Willebrand's disease antigen (Factor VIII-like antigen, vW-Ag) are biologically linked, and it has been suggested that they reside on the same molecule. However, insolubilized human isoantibody to VIII(C) and rabbit antiserum containing antibodies to VIII(C) and vW-Ag differentially bind and remove these entities from plasma, thus physically ...
متن کاملProgress of in vitro factor VIII coagulant activity from 0 to 8 hours after reconstitution
BACKGROUND Continuous infusion of factor VIII (FVIII) is a more cost-effective method for treating hemophilia A than intermittent bolus injection. However, there is currently no specific data in Korea about the progress of in vitro FVIII coagulant activity (FVIII:C) after reconstitution from its lyophilized form. METHODS Three commercial FVIII concentrate products (two recombinant FVIII and o...
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ژورنال
عنوان ژورنال: Blood
سال: 1982
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v59.3.594.594