Monoclonal antibodies to glutamic acid decarboxylase.

Monoclonal antibodies to glutamic acid decarboxylase.

Five monoclonal antibodies that recognize chicken brain glutamic acid decarboxylase (GAD) have been selected and designated GAD-1 to -5. GAD-1 to -5 were selected on the basis of their ability to immunoprecipitate active GAD from crude brain extracts. GAD-1 recognizes an epitope that is conserved in many vertebrates; the epitope recognized by GAD-5 is restricted to the chicken. Radioimmunoassay...

Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase.

Immunoaffinity columns are prepared from the monoclonal antibody (MAb) GAD-1. These columns are used to enrich glutamic acid decarboxylase (GAD) from the cytosolic fraction of rat brain homogenates and from Triton X-100 extracts of the brain membrane fraction. In each case enzyme activity is enriched over 400-fold. The immunopurified fractions were analyzed by SDS-PAGE. Fractions purified from ...

INVESTIGATION ON ANTI-GLUTAMIC ACID DECARBOXYLASE ANTIBODIES IN TYPE I DIABE TES MELLITUS

Antibodies directed against the enzyme glutamic acid decarboxylase (GAD) are believed to be the main cause of destruction of pancreatic islet cells in type I (insulin dependent) diabetes mellitus. The enzyme was found both in the brain and pancreatic beta cells. Although similarities in identity of GAD in human and rat brain have been demonstrated, little is known about the interaction betw...

Association of Paraneoplastic Neurological Disorders With Glutamic Acid Decarboxylase Antibodies.

Antibodies against glutamic acid decarboxylase: prevalence in neurological diseases.

UNLABELLED High prevalence of autoantibodies against glutamic acid decarboxylase (GAD-Ab) in stiff man syndrome (SMS) not only helps diagnosis, but also suggests immune mediated impairment of GABAergic functions. However, the presence of GAD-Ab has also been reported in other neurological syndromes. Therefore the prevalence of GAD-Ab was investigated in SMS, progressive encephalomyelitis with r...