Molecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens
نویسندگان
چکیده
منابع مشابه
Molecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens.
In this manuscript, recent research from this laboratory into physiological and molecular aspects of heavy metal (Zn) transport in the hyperaccumulating plant species, Thlaspi caerulescens is reviewed. This research is aimed at elucidating the processes that underlie the accumulation of extraordinarily high levels of Zn in the T. caerulescens shoot (up to 3% Zn dry wt.) without any associated t...
متن کاملThe molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens.
An integrated molecular and physiological investigation of the fundamental mechanisms of heavy metal accumulation was conducted in Thlaspi caerulescens, a Zn/Cd-hyperaccumulating plant species. A heavy metal transporter cDNA, ZNT1, was cloned from T. caerulescens through functional complementation in yeast and was shown to mediate high-affinity Zn(2+) uptake as well as low-affinity Cd(2+) uptak...
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Hyperaccumulators store most of the accumulated metal in the vacuoles of large leaf epidermal cells (storage cells). For investigating cadmium uptake, we incubated a protoplast mixture obtained by digestion of leaves of Thlaspi caerulescens (Ganges ecotype) with a Cd-specific fluorescent dye. A fluorescence kinetic microscope was used for selectively measuring Cd-uptake and photosynthesis in di...
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Laboratoire de Physiologie et Génétique Moléculaire des Plantes, Université Libre de Bruxelles, Campus Plaine (CP 242) Bd du triomphe, B-1050 Brussels, Belgium.
متن کاملCellular compartmentation of zinc in leaves of the hyperaccumulator thlaspi caerulescens
Cellular compartmentation of Zn in the leaves of the hyperaccumulator Thlaspi caerulescens was investigated using energy-dispersive x-ray microanalysis and single-cell sap extraction. Energy-dispersive x-ray microanalysis of frozen, hydrated leaf tissues showed greatly enhanced Zn accumulation in the epidermis compared with the mesophyll cells. The relative Zn concentration in the epidermal cel...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2000
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jexbot/51.342.71