Molecular identification of pathogenic bacteria and PCR specific primer design

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

PCRTiler: automated design of tiled and specific PCR primer pairs

Efficiency and specificity of PCR amplification is dependent on several parameters, such as amplicon length, as well as hybridization specificity and melting temperature of primer oligonucleotides. Primer design is thus of critical importance for the success of PCR experiments, but can be a time-consuming and repetitive task, for example when large genomic regions are to be scanned for the pres...

متن کامل

Specific PCR Primer and Probe Design Based on Spanning Networks

While the amount of molecular data that can be routinely generated continues to increase, advances in the methods of analysis to handle this quantity of data have not kept pace. One area of research which could greatly benefit from new approaches is the design of specific probes and PCR primers. Here we generated spanning networks (1) for phytopathogenic Pseudomonas strains from seven species, ...

متن کامل

PCR Primer Design

To make PCR a specific, efficient and cost effective tool for researchers and clinicians the most important aspect is oligonucleotide primer design. This review discusses various aspects of primer design. Advice is provided for optimal design and the role of bioinformatic tools is highlighted. The authors discuss theoretical considerations and compare computational and experimental studies.

متن کامل

Homolog-specific PCR primer design for profiling splice variants

To study functional diversity of proteins encoded from a single gene, it is important to distinguish the expression levels among the alternatively spliced variants. A variant-specific primer pair is required to amplify each alternatively spliced variant individually. For this purpose, we developed a new feature, homolog-specific primer design (HSPD), in our high-throughput primer and probe desi...

متن کامل

Primer Design with Specific PCR Product using Particle Swarm Optimization

Before performing polymerase chain reactions (PCR), a feasible primer set is required. Many primer design methods have been proposed for design a feasible primer set. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specifi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: e-Journal BUDIDAYA PERAIRAN

سال: 2013

ISSN: 2684-7396,2337-3768

DOI: 10.35800/bdp.1.3.2013.2733