Molecular identification of non-lactic acid bacteria isolated on MRS medium and associated to the production of biogenic amines in adjuevan, a fermented fish of Côte d’Ivoire

Biogenic amines (BAs) are important nitrogenous compounds formed mainly by decarboxylation of amino acids or amination and transamination aldehydes ketones. That have been detected in adjuvant, a fermented fish very used as condiments Côte d’Ivoire. The aim this study was to identify non-lactic acid bacteria (non-LAB) populations isolated adjuevan on MRS agar producing biogenic amines. Thus, non-LAB were agar, then their production highlighted Niven agar. Finally, the isolates which produce identified sequencing 16S DNA region. results showed that able grow Their charges obtained between 1.3x103 1.95x105 UFC/g. Those also during storage at ambient temperature 4°C until six weeks. prevalence from 8.8 100% 57% produced with Chloroscombrus chysurus. This 21 50% 55% Galeoides decadactylus weeks storage. However, low Thunnus thynnus. species found molecular identification Staphylococcus saprophyticus, kloosi, gallinarum, arlettae, cohnii, Bacillus subtilis subsp. subtilis, Pseudomonas sp., Enterococcus faecalis, Streptococcus pyogènes Enterobacter sichuanensis. saprophyticus most amines-producing over 71% strains.