Molecular Diagnostics in Pathology: Time for a Next-Generation Pathologist?

Next-generation sequencing: a new revolution in molecular diagnostics?

In 1980, Fred Sanger and Walter Gilbert were awarded the Nobel Prize in Chemistry for discovering novel ways for sequencing nucleic acids. In 2003, the human genome sequence was published, an effort that involved more than 3000 scientists from 6 countries. The work took 13 years to complete, at a cost of nearly $3 billion. Only 6 years later, nucleic acid sequencing technologies have advanced t...

The anatomic pathologist meets molecular pathology.

With the publication in this issue of " Stuck Between a Scalpel and a Rock, or Molecular Pathology and Legal-Ethical Issues in Use of Tissues for Clinical Care and Research: What Must a Pathologist Know? " by Dry et al, 1 we launch a new series for the American Journal of Clinical Pathology in a forward-looking celebration of the Journal's commitment to readers' education. It will include invit...

Application of Next-generation Sequencing in Clinical Molecular Diagnostics

Next-generation sequencing (NGS) is the catch all terms that used to explain several different modern sequencing technologies which let us to sequence nucleic acids much more rapidly and cheaply than the formerly used Sanger sequencing, and as such have revolutionized the study of molecular biology and genomics with excellent resolution and accuracy. Over the past years, many academic companies...

Next-Generation Pathology.

The field of pathology is rapidly transforming from a semiquantitative and empirical science toward a big data discipline. Large data sets from across multiple omics fields may now be extracted from a patient's tissue sample. Tissue is, however, complex, heterogeneous, and prone to artifact. A reductionist view of tissue and disease progression, which does not take this complexity into account,...

Next-generation pathology.