Molecular Detection of the Toxin Gene toxA in Pasteurella multocida Isolated from Calves
نویسندگان
چکیده
منابع مشابه
Molecular Characterisation of Pasteurella Multocida Strains Isolated from Calves by Pulsed-field Gel Electrophoresis
Seventy-two Pasteurella multocida strains collected from calves with bronchopneumonia were examined. An analysis of the polymorphism of DNA restrictive fragments with the use of the pulsed-field gel electrophoresis technique of contour-clamped homogeneous electric field electrophoresis was made. Philogenetic relatedness among the examined strains was calculated by the unweighted pair-group meth...
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This study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty Holstein calves with dairy calf pneumonia from dairy farms of Mashhad Suburb between September 2002 and August 2003 . The most common micro-organisms isolated were Pasteurella multocida 80 (61.54%), Mannheimia haemolytica 41 (31.54%), Bacillus sp. 15 (11.54%), Staphy...
متن کاملMolecular Characterization of Pasteurella multocida Isolated from Rabbit in Egypt
Objective: The study aimed to molecular characterization of Pasteurella multocida local strain using Enterobacterial Repetitive Intergenic Consensus (ERIC), 13 virulence genes and the outer membrane protein (OmpH) gene. Also, development of diagnostic markers by multiplex PCR from investigated genes. Methodology: The ERIC-PCR was used for the detection of the finger print of Pasteurella multoci...
متن کاملPrevalence of different OmpH-types among Pasteurella multocida isolated from lungs of calves with respiratory problems.
Pasteurella multocida is among the most important respiratory pathogens of cattle. Outer-membrane protein (OmpH) constitutes an essential bacterial antigen and is well studied in avian bacterial strains. Studies on isolates from cattle with signs of respiratory disease caused by Pasteurella multocida serotypes A and D have not yet been covered in the literature. The objective of this study was ...
متن کاملAssociation of Pasteurella multocida toxin with vimentin.
To help understand the molecular mechanisms of Pasteurella multocida toxin (PMT) action, we searched for a cellular protein interacting with PMT. The ligand overlay assay revealed a 60-kDa cellular protein that binds to a region from the 840th to 985th amino acids of the toxin. This protein was identified as vimentin by peptide mass fingerprinting. The N-terminal head domain of vimentin was fur...
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ژورنال
عنوان ژورنال: Journal of Animal Health and Production
سال: 2020
ISSN: 2308-2801
DOI: 10.17582/journal.jahp/2021/9.s1.14.19