Molecular Detection of Hepatitis B Virus Among HBsAg Non-Reactive Blood of Donors

Despite all blood donations being tested routinely for HBsAg as a clinical marker of transmissible HBV, cases post-transfusion hepatitis B virus infection are still reported because molecular studies using Polymerase Chain Reaction (PCR) not available Transfusion Transmissible Infection (TTIs) testing. In this study, we sought to use the PCR technique re-screen donated that had already been proven be non-reactive with rapid diagnostic testing and ELISA. One hundred eighty-five samples were obtained from proportion deposited at bank Federal Medical Center, Birnin-Kebbi, Nigeria. Socio-demographic parameters such age group, status, ethnicity, occupation, group PCV donors' records. Nested was employed detect HBV DNA. Furthermore, genotyping performed determine genotypes in positive genotype-specific primers. Of 185 donors, it observed five (2.7%) population HBV. is more common among people aged 18–30, singles, Hausa, self-employed. addition, genotype E. This study suggests need complement antibody-based tests DNA effective screening consequent safe transfusion. Keywords: Hepatitis Virus; HBsAg; PCR;