Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI
نویسندگان
چکیده
منابع مشابه
Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI.
The highly specific restriction endonucleases Sa1I and BamI produce DNA fragments with complementary, cohesive termini that can be covalently joined by DNA ligase. The Escherichia coli kanamycin resistance factor pML21 has one SalI site, at which DNA can be inserted without interfering with the expression of drug resistance or replication of the plasmid. A more convenient cloning vehicle can be...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.5.1537